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  • A beneficial Advanced Metabolomics Workshop is hosted by the ACGT at the University of Pretoria for metabolomics researchers from all over South Africa

    20th March 2019

    The African Centre for Gene Technologies (ACGT), the University of Johannesburg and the University of Pretoria organized the 2019 Advanced Metabolomics Workshop that was held at the University of Pretoria from 13-15th March 2019.  The workshop provided a capacity building platform to address key issues and challenges in the field of metabolomics.

    The workshop was facilitated by this panel of elite metabolomics experts. The facilitators were Dr Reza Salek (International Agency for Research on Cancer, Lyon, France), Dr Karl Burgess (University of Edinburgh, Scotland, UK), Dr Fabien Jourdan (INRA Toulouse, France), Dr Justine Bertrand-Michel (INSERM Toulouse, France), Dr Naomi Rankin (University Hospital Wishaw, Scotland, UK), Dr Jasper Engel (Wageningen University & Research, The Netherlands) and Dr Fidele Tugizmana (University of Johannesburg, RSA).

    Useful tools

    This workshop covered advanced metabolomics topics using a combination of lectures, interactive round table discussions and computer-based practical sessions. The topics covered in the workshop included applications of metabolomics (clinical, plant/agricultural, industrial), LC-MS/MS/NMR metabolite identification, simple and multivariate statistics, data standards and metabolomics resource, network-oriented metabolomics data mapping, advanced data analysis, lipidomics, metabolomics networks and using fragmentation to enhance metabolite IDs. Throughout the three days, the delegates also had a chance to ask project-specific questions and advice from the with facilitators.

    The participants were from the ACGT partner institutions, as well as outside institutions including previously disadvantaged research institutions. There were participants from University of Pret

    Hands on training

    oria, University of Johannesburg, University of the Witwatersrand, Agricultural Research Council, Council for Scientific and Industrial Research, North-West University, University of South Africa, Tshwane University of Technology, University of the Free State, Walter Sisulu University, University of Cape Town and the University of Venda. Delegates exhibited different scientific backgrounds and had the chance to get to know each other through two-minute elevator-type flash presentations on the first day of each workshop.

    Some fun was had too!

    The ACGT would also like to wish all the participants of the workshops all the luck with their work and future in metabolomics. Furthermore, the ACGT sends much deserved gratitude to all the members of the organizing committee; Mr Molati Nonyane, Mrs Itseng Malao, Dr John Becker, Dr Fidele Tugizimana, and the European visitors for all of their efforts in making this event a success. The ACGT would also like to extend their gratitude to the sponsors of this event in Thermo Fisher Scientific and the Embassy of France in South Africa.

    The delegates and facilitators

    If you would like to view most of the pictures from this event, please visit our facebook page:

    https://www.facebook.com/ACGT.biotec/

    For any metabolomics-related capacity building and networking queries, kindly contact Mr Molati Nonyane, ACGT Liaison Scientist (az.ca1558876645.pu@e1558876645nayno1558876645n.ita1558876645lom1558876645, 0124206139).

  • Metabolomics South Africa (MSA) is officially launched during a Metabolomics Symposium in Pretoria

    Metabolomics South Africa (MSA) is officially launched during a Metabolomics Symposium in Pretoria
    20th March 2019

    The 2019 Metabolomics symposium was held at the Innovation Hub from 11-12th March 2019. This symposium was aimed at principal investigators, postgraduate and PhD students currently performing or planning to work in the metabolomics field. This event was an opportunity for both local and international metabolomics researchers to share their findings, to network and identify synergies so as to foster further collaboration amongst metabolomics researchers within SA and across Europe. This symposium was mainly a platform to officially launch Metabolomics South Africa (MSA). MSA is a non-profit organization seen as an opportunity to promote and improve the profile of metabolomics research and technology in South Africa, foster networking, training, capacity building, information sharing, mentoring, career opportunities, leadership training and professional development. The symposium was a first official event hosted by MSA with the assistance of its partner, the ACGT.

    The first day of the symposium was themed “Metabolomics, where are we?”. Topics discussed throughout the day included the overview of metabolomics in South Africa, good metabolomics data, role of metabolomics in health sciences, lipidomics and plant metabolomics. The discussions on the second day of the symposium were under the theme “Metabolomics, informatics, statistics and applications” and covered topics such as metabolomics networks, statistical models, NMR metabolomics and lipidomic profiling.  At the end of each day, attempts were made to finish off with round table discussions to address burning issues or questions on the day’s talks as well as other issues regarding to metabolomics or MSA.

     

    It is worth mentioning several key speakers that participated and contributed to the symposium. The speakers mentioned below are all key contributors to the field of Metabolomics and we are thankful that they shared their work and experiences. These were the presenters at the event in no specific order:

    • Dr Reza Salek is currently with the International Agency for Research on Cancer, Lyon, France. Dr Salek has extensive experience in metabolomics research in academia, industry and research institutes with extensive metabolomics knowledge, both analytical and data handling. Dr Salek has worked in clinical trial settings and is interested in setting up workflow infrastructures for metabolomics data handling and analysis using cloud computing.
    • Dr Karl Burgess is from the University of Edinburgh, Scotland, UK. Most of Dr Burgess’s workfocuses on the use of mass spectrometry in biomedical research, particularly in the understanding of infectious disease. This multidisciplinary research area builds on his fruitful collaborations with cell biologists, engineers, bioinformaticians, instrumentation developers and clinicians.
    • Dr Fabien Jourdan is a research scientist at the French National Research Institute for Agricultural Research (INRA). Dr Jourdan develops computational methods to study human genome-scale metabolic network aimed at retrieving parts of the metabolism affected by genetic or environmental perturbations. These methods are mainly applied to toxicology and human health.
    • Dr Naomi Rankin is from the University Hospital Wishaw, Scotland, UK. Dr Rankin’s research interests focus on the use of NMR for metabolite profiling and advanced lipoprotein profiling of serum and plasma samples. She is particularly interested in the use of these methods in epidemiology/clinical trials, especially with a view towards translation of NMR molecular profiling approaches into the clinic.
    • Dr Jasper Engel is a researcher at Biometris at Wageningen University & Research. Dr Engel’s primary research interests are in applied statistics. He is interested in the development and careful evaluation of statistical approaches for processing and analysis of complex high-dimensional data sets, and their application in high-dimensional chemical, biological or medical problems. Over the last several years his research has mainly focused on method development for analysis of metabolomics experiments.
    • Dr Justine Bertrand-Michel is the co-director of the MetaToul Lipidomic facility at INSERM in Toulouse, France. Dr Bertrand-Michel is a lipidomics expert and she was invited to this symposium because of her work in lipidomics a field that most people in metabolomics have shown a keen interest in.
    • Dr Fidele Tugizimana is the chairman of MSA, a research scientist at the University of Johannesburg, a specialist scientist in the international R&D management of the Omnia (Pty) Ltd company (SA) and a scientific consultant in the L.E.A.F. Pharmaceuticals LLC (USA & Rwanda). Dr Tugizimana applies metabolomics approaches in plant-environment interactions (involving abiotic/biotic stresses, beneficial microorganisms, etc.).
    • Prof Du Toit Loots is the secretary of MSA and a key member of the national metabolomics platform based at the North-West University in Potchefstroom. Prof Loots has made a substantial contribution to the advancement of metabolomics, by means of developing much of the published methodology and applications in terms of identifying new markers for better disease characterization, diagnostics and treatment since 2002.
    • Dr Aurelia A. Williams is the deputy secretary of MSA and a senior lecturer at the biochemistry department of the North-West University. Her research interests include characterizing the impact of molecular traits, metabolism and the immune response on disease pathogenesis. She believes metabolomics will serve as a tool in better characterizing infectious diseases such as HIV/AIDS, HIV-associated comorbidities, virus-host interactions, treatment response mechanisms and pathogenesis-associated phenotypes.
    • Dr Zandile C. Mlamla is a committee member of MSA and a post-doctoral research fellow at the Institute of Infectious Disease and Molecular Medicine (IDM), University of Cape Town (UCT). Her research is currently aimed towards the validation of urinary biomarkers of Tuberculosis Disease. She continues to gain experience and expertise in the cutting-edge field of MS-based lipidomics.

    The future of South African metabolomics will be in great hands if the student sessions over the two days are anything to go by. On day one, the student presenters were Mr Efficient Ncube from the University of Johannesburg, Mr Emile Jansen van Rensburg and Ms Monique Combrink both from the North-West University. The student session on day two was by three students from the North-West University in Ms Karin Terburgh, Mrs Zinandre´ Stander and Christiaan van Zyl. The students presented their ongoing work and they received great contributions from the people that attended the symposium in the form of question and suggestions.

    Well over 140 delegates from over 25 institutions attended this symposium. They consisted of principal investigators, post-doctorates, researchers, post graduate students and several industry players. The hope is that most of them will register as member of MSA and take an active role in the growth of MSA and metabolomics in general.  The ACGT and MSA would like to thank all the delegates who registered to attend this event as well as all the members of the organizing committee; Mr Molati Nonyane, Mrs Itseng Malao, Dr John Becker, Dr Fidele Tugizimana, MSA Committee and the European visitors for all of their efforts in putting together this event. The ACGT and MSA would also like to extend their gratitude to the sponsors of this event in Shimadzu, Thermo Fisher Scientific, Microsep/Waters and the Embassy of France in South Africa. Please look out for more MSA and/or ACGT events similar to this symposium in the future.

    If you would like to view more of the pictures from this event, please visit our facebook page:

    https://www.facebook.com/ACGT.biotec/

  • The ACGT hosts a successful whole transcriptome sequencing data analysis workshop

    The ACGT hosts a successful whole transcriptome sequencing data analysis workshop
    26th February 2019

    The Africa Centre for Gene Technologies (ACGT), in conjunction with the University of Zurich, University of Pretoria and the Agricultural Research Council, hosted a successful whole transcriptome sequencing (RNA sequencing or RNASeq) data analysis workshop for researchers from the 1st to the 8th of February 2019. The workshop was held at the Hatfield campus of the University of Pretoria.

    RNA sequencing aims to unravel the sum of all transcripts in an organism at any given moment in time and can give important clues to changes occurring in an organism following a variety of environmental cues or life stage transitions. The workshop started with two days of introductory courses in Linux and R. The introductory courses were facilitated by Professor Fourie Joubert from the University of Pretoria and Dr Rian Pierneef from the Agricultural Research Council. These courses offered the delegates basic command line skills needed to manipulate RNA sequencing data.

    Prof. Mark Robison sharing valuable knowledge and skills with the delegates

    The two days of introductory courses were followed by a four-day interactive and hands-on RNA Sequencing workshop. The workshop was facilitated by renowned experts and academics from the Institute of Molecular Life Sciences at the University of Zurich in Switzerland: Professor Mark Robinson and Dr Simone Tiberi. Professor Mark Robinson is involved in research that applies statistical methods and data science to experimental data with biological applications within the context of genomics data types. Dr Simone Tiberi is a post-doctoral research fellow who is working on the development of cutting-edge statistical methods in bioinformatics, mostly for bulk and single-cell RNASeq data.

     

    Dr Simone Tiberi assisting the delegates in of of the hands-on sessions

    The workshop covered theoretical aspects of RNA sequencing such as new technologies, applications, experimental design, batch effects, dimension reduction, clustering, quality control, limma, normalization quantification and differential expression, among other things. There were also one-on-one sessions with delegates to address their specific queries or to clarify any issues that may have arisen. The delegates hailed mostly from the ACGT partner institutions; University of Pretoria, University of Johannesburg, University of the Witwatersrand and the Agricultural Research Council. The feedback from the delegates was very positive in how the course was designed, organized and conducted. The ACGT is hopeful that the course will be beneficial to all the researchers that came to the workshop and to those that work close with them.

    The ACGT and all of its partners would like to extend enormous gratitude to Professor Mark Robinson, Dr Simone Tiberi, Dr Rian Pierneef and Professor Fourie Joubert for sharing their time and expertise with the attendees. The ACGT would also like to thank Professor Fourie Joubert and Mrs Itseng Malao for all of their assistance in organizing this event. The Centre wishes all the participants the best of luck with applying their newly learned skill to their work.

    Who said learning isn’t fun?

     To view some additional pictures from the event please visit our facebook page.

    https://www.facebook.com/media/set/?set=a.2516672451738564&type=1&l=b3ae88d048

  • Whole Transcriptome Sequencing Data Analysis workshop 01 – 08th February 2019 University of Pretoria

    21st January 2019

    RNA Seq aims to unravel the sum of all transcripts in an organism at any given moment in time and can give important clues to changes occurring in an organism following a variety of environmental cues or life stage transitions. The ACGT, in conjunction with the Universities of Zurich, Pretoria and the Agricultural Research Council, will host a whole transcriptome sequencing (RNA sequencing or RNA Seq) data analysis workshop for researchers in Pretoria during the first week of February 2019. In preparation for the workshop, there will also be introductory sessions to Linux and R for those accepted to attend the workshop.

    Important dates:

    ·      01 February 2019 (Friday) – Introduction to Linux
    ·      04 February 2019 (Monday) – Introduction to R
    ·      05-08 February 2019 (Tuesday to Friday) – RNA Seq WorkshopTopics:

    ·      Theoretical aspects (technologies and applications, experimental design, batch effects, dimension reduction, clustering, QC, limma, normalization)
    ·      Environment setup (Organizing files, running QC on datasets)
    ·      Quantification
    ·      Differential expression
    ·      Advanced topics (Geneset testing, single cell pre-processing, workflow)
    ·      One-on-one sessions with delegates to address their specific queries or to clarify any issues that may have arisen.

    The Facilitators:

    The workshop will include a mix of lectures and hands-on practical sessions facilitated by renowned and expert academics. The facilitators will include:
    ·      Professor Mark Robinson: Professor of Statistical Genomics at the Institute of Molecular Life Sciences at the University of Zurich in Switzerland – Research interests are in the application of statistical methods and data science to experimental data with biological applications within the context of genomics data types.
    ·      Doctor Simone Tiberi: Post-Doctoral Research Fellow, also from the University of Zurich, who works on on the development of cutting-edge statistical methods in bioinformatics, mostly for bulk and single-cell RNA-seq data.
    ·      Professor Fourie Joubert: Bioinformaticist and Director of the Centre for Bioinformatics and Computational Biology at the University of Pretoria.
    ·      Doctor Rian Pierneef: Bioinformatics Research Scientist at the Biotechnology Platform of the Agricultural Research Council.Application Process:

    Should you be interested in attending this course, please provide your details and your reasons for attending the course before noon on Wednesday, 23 January 2019.

    Please use this link to apply:

    https://www.surveymonkey.com/r/RNASeqWorkshopACGT

    Kindly be as descriptive as possible in answering questions as selections will be based on the answers you provided. If you know someone else who stands to benefit from this course, kindly forward this email to the relevant party. Please note that incomplete applications will not be considered. We look forward to receiving your applications.

    On behalf of the organising committee

    Molati Nonyane

    Liaison Scientist 

    African Center for Gene Technologies

    Experimental Farm,  South Street

    University of Pretoria

    E-mail: az.ca1558876645.pu@e1558876645nayno1558876645n.ita1558876645lom1558876645

    Tel: +27 12 420 6139/ +27 71 887 0438

    www.acgt.co.za

  • Metagenomics researchers receive much needed training in using R for data visualization

    Metagenomics researchers receive much needed training in using R for data visualization
    28th November 2018

    The African Centre for Gene Technologies (ACGT) reached out to numerous metagenomics researchers throughout the country and determined that most of their capacity-building needs were data orientated. A large number of metagenomics stakeholders generate huge amounts of data from samples derived from diverse environments. The stakeholders felt they were not getting maximum value out of vast amount of metagenomics data they have gathered due to limitations in skills to effectively analyse and interpret the data. The ACGT decided to assist in addressing this issue by organizing multiple training initiatives starting with a metagenomics data visualization & data interpretation workshop.

    Delegates during one of the sessions

    The metagenomics data visualization & data interpretation workshop ran from 12-14th November 2018 at the University of Pretoria and was facilitated by a local expert, Dr Rian Pierneef. Dr Pierneef is a bioinformatics research scientist at the Biotechnology Platform of the Agricultural Research Council. Dr Pierneef specializes in biological data analysis and has conducted numerous research studies on prokaryotes in the agricultural environment. The three-day workshop focused on using R for metagenomics data visualization. R is a programming language and free software environment for statistical computing and graphics. The R language is widely used among statisticians and data miners for developing statistical software and data analysis. The workshop started with introducing R to the workshop attendees and then proceeded to direct the attendees through the use of R packages for metagenomics analysis and data visualization.

    Data visualization
    Learning R

    The delegates were from different research backgrounds and hailed mostly from the ACGT partner institutions; University of Pretoria, Agricultural Research Council and Council for Scientific and Industrial Research. The lectures were kept as relaxed as possible with open discussions and computer-based practical sessions. Through the interactive sessions, the attendees were able to obtain advice and assistance that was specific to their individual projects. There was a general sense from the course evaluations that the course was relevant, practical and beneficial. The ACGT and all of its partners would like to extend enormous gratitude to Dr Rian Pierneef for sharing his time and expertise with the attendees. The ACGT would also like to thank Professor Fourie Joubert and Mrs Itseng Malao for all of their assistance in organizing this event. The ACGT wishes all the participants the best of luck with applying their new earned skill to their work. Due to limited space, not all of the applicants could be hosted but the ACGT encourages those that were not placed this year to keep an eye out for future courses.

    For any other capacity building and networking queries, kindly contact Mr Molati Nonyane, ACGT Liaison Scientist (az.ca1558876645.pu@e1558876645nayno1558876645n.ita1558876645lom1558876645, 012 420 6139).

    Here is a link to some of the images taken during the event:

    https://www.facebook.com/media/set/?set=a.2339127189493092&type=1&l=ebfe58f9b5

     

     

     

  • ACGT Metabolomics Workshops build national biotechnology capacity

    ACGT Metabolomics Workshops build national biotechnology capacity
    4th April 2018

    One of the objectives of the African Centre for Gene Technologies (ACGT) is to improve the advanced biotechnology skills level of scientists in South Africa. The quality of the work done locally should match, or even exceed, related work from any part of the world and the best way to do that is through continuous capacity building efforts.

    To this end, the ACGT recently facilitated two metabolomics workshops to provide a platform to address key issues and challenges in the field of metabolomics, including subsequent data analyses. The two workshops were meant for different audiences and were held at the University of Pretoria. The first was an Introductory Workshop that ran from the 12th to the 14th of March 2018. This event was aimed at those who have limited knowledge of metabolomics techniques and applications. The second, an Advanced Workshop, subsequently ran on the 15th and 16th of March 2018 and covered more advanced topics in the field. The latter workshop was intended for those with existing metabolomics knowledge and already working with some metabolomics techniques, or are actively engaged in data analyses emanating from metabolomics experiments.

    Both workshops were designed to include a mixture of lectures, interactive round-table discussions and computer-based practical sessions. The Introductory Workshop covered topics that ranged from experimental design, introduction to techniques, applications of metabolomics, analysis of metabolomics data, metabolite identification, statistics and metabolomics data interpretation. The Advanced Metabolomics Workshop focused on advanced data analysis, quantitative metabolomics, metabolomics networks and data sharing.


    The participants hailed from the ACGT partner institutions, as well as institutions outside the partnership, including previously disadvantaged research institutions (University of Pretoria, University of Johannesburg, University of the Witwatersrand, Agricultural Research Council, Council for Scientific and Industrial Research, North-West University, University of South Africa, Tshwane University of Technology and the Central University of Technology (Free State). Delegates exhibited different scientific backgrounds and had the chance to get to know each other through two-minute elevator-type flash presentations on the first day of each workshop.

    The workshops were facilitated by a team of renowned metabolomics experts, both local and international. The local facilitators included Dr Fidele Tugizimana and Professors Ian Dubery and Paul Steenkamp from the University of Johannesburg. The international facilitators included Dr Reza Salek from the University of Cambridge, Dr Jos Hageman from Wageningen University, Dr Fabien Jourdan from the French National Research Institute for Agricultural Research, and Drs Naomi Rankin and Karl Burgess (both from the University of Glasgow).

    Following the brief ice breaker presentations by the individual participants on their backgrounds and areas of interest, the workshops then proceeded onto a variety of subjects that influence and shape metabolomics research. Web tools and practical applications of metabolomics were demonstrated, as well as hands-on exercises that focused on data analysis and interpretation. The sessions were kept as interactive as possible to create a relaxed environment where the participants could ask questions pertaining to their own work.

    The ACGT and all of its partners would like to extend enormous gratitude to all those who contributed to making these workshops a success, including the facilitators who again took a lot of time out of their busy schedules to contribute to capacity building in South Africa. The ACGT would also like to wish all the participants of the workshops all the luck with their work and future in metabolomics.

    There has since been positive feedback from the participants of both workshops. There was a general sense from the workshop evaluations that the workshops were informative, practical and interesting. The participants enjoyed interacting with the expert facilitators from different parts of the globe. They felt that the facilitators were knowledgeable and showed willingness to help. Moving forward, there are plans to adjust the duration of the course to increase subject coverage, as well as to include additional practical sessions. Due to limited space, the Centre could not host everyone that applied, but the ACGT encourages those that were not placed this year to keep an eye out for future workshops.

    The workshops represent one of the means towards building a sense of community- further building on a stakeholder session that was facilitated earlier in 2018 towards the establishment of a more formal grouping of metabolomics stakeholders. More news on the establishment of an association to follow soon.

    For any metabolomics-related capacity building and networking queries, kindly contact Mr Molati Nonyane, ACGT Liaison Scientist ( az.ca1558876645.pu@e1558876645nayno1558876645n.ita1558876645lom1558876645,
    012 420 6139 )

  • GMASSURE Launch and Awareness Raising Symposium

    GMASSURE Launch and Awareness Raising Symposium
    7th July 2014

    The GMASSURE Launch and Awareness Raising Symposium was held on the 2nd and 3rd July 2014 in Centurion (Gauteng, South Africa). Presentations from the event can be accessed below.

    Presentations Day 1:

    1. Welcome, Overview and Introduction to GMASSURE Activities_ Dr John Becker
    2. Importance of Biosafety Regulation and Risk Analysis_ Dr Alex Owusu-Biney
    3. A regulator’s perspective of GM biosafety- why, what and who_ Mr Ben Durham
    4. Crop biosafety in South Africa- problems, solutions, level of awareness and the role of Biosafety SA_Dr Hennie Groenewald
    5. Agricultural Biotechnology in the Bioeconomy – the importance of GM crops in Southern Africa_Dr Manshree Jugmoham-Naidu
    6. Experiences in sub-Saharan Africa with GM crop risk communication_Dr Dennis Ndolo Obonyo

    Presentations Day 2: