Facilities - Molecular and Biomedical Technologies


  • Chemical synthesis and analysis

    13th Sep 2012

    The Molecular Technologies facility provides access to expertise and modern technologies for the generation and expansion of small compound libraries, the development of analogue series, and the identification, characterisation and quantification of specific compounds and natural extracts through its broad range of analytical expertise.

    Equipment:

      • State-of-the-art technologies for chemical synthesis (Chemspeed robotic and microwave synthesis platforms)
      • Semi-preparatory and preparatory chromatography (including TLC, HPLC, CCC)
      • Associated detection methods (UV/VIS, fluorescence, MS)
      • NMR technologies and in-silico platforms for the identification of molecules and the elucidation of their 3D structure.

    The facilities capabilities include:

    Chemical library management:

    • Compound management and hosting
    • Miniaturization
    • Natural and synthetic collections
    • Library generation based on specific chemotypes

    For small and natural compounds:

      • Identification and isolation from complex matrices
      • Natural and simplified ADME model
      • Chemical modifications
      • Bioactivation/inactivation models
      • In-silico modelling and design

    Analysis of small and large molecules:

      • Biomarker ID and quantification in products (QC)
      • Biomarker ID and quantification in complex matrices (PK, Clinical studies)
      • Characterisation and quantification of specific analytes
      • Purity determination
      • Method development and validation

    Contact:
    Library and compound synthesis
    Dr Edwin MMutlane
    Molecular Technologies
    CSIR Biosciences
    Tel: 012 841 4282
    Email: az.oc1569109294.risc1569109294@enal1569109294tuMME1569109294

    Compound analysis
    Dr Paul Steenkamp
    Molecular Technologies
    CSIR Biosciences
    Tel: 012 841 3260
    Email: az.oc1569109294.risc1569109294@pmak1569109294neetS1569109294P1569109294

  • Drug and cosmetics screening

    13th Sep 2012

    The pharmacology and bioassay facility designs, develops and validates pharmacological assays for drug benefit and risk assessment, which are applied in biomolecular screening campaigns of small to medium sized chemical and extract libraries.

    Equipment:

    • State-of-the-art liquid handling and high-throughput robotic assay facilities
    • High-end spectrophometry for absorbance/fluorescence measurements
    • Realtime PCR
    • Agilent Bioanalyzer analysis for RNA, DNA and protein integrity

    Services:

    In vitro models for drug benefit and risk assessment include:

    Pathogens and infectious disease

      • Antiviral screens including HIV pseudovirus
      • Malaria (conventional and transmission blocking screens)
      • Antimicrobial screens
      • Antifungal screens
      • Drug signature profiling

     

    Mammalian cell tissue culture

      • Cancer and oncology screens
      • Drug permeability
      • Drug cytotoxicity screening
      • Drug signature profiling

    The facility also develops and applies assays for cosmeceuticals testing. In vitro models include:

    Enzyme and cell-based assays

    • Elastase, collagenase, hyaluronidase screening for activity inhibition
    • Elastin and collagen screening for protein production

    Targeted and whole genome assays

    • Cell-based TaqMan and microarray expression profiling of molecular biomarkers specific to youthful skin and ageing

    Contact:
    Ms Natasha Kolesnikova / Dr Malefa Tselanyane
    Biomedical Technologies
    Tel: 012 841 4735 / 012 841 4045
    Email: az.oc1569109295.risc1569109295@avok1569109295insel1569109295oKN1569109295 / az.oc1569109295.risc1569109295@enay1569109295nales1569109295tm1569109295

  • Biomarker discovery/Validation and biomolecule analysis

    13th Sep 2012

    The biomarker discovery/validation and biomolecule analysis facility characterises and quantifies specific compounds through a broad range of analytical services.

    Equipment:

    Electrophoresis
    Ettan IPGphor I/II IEF (GE Amersham)
    Rotofor Cell (Bio-Rad)
    Dalt II Separation Unit (GE Amersham)
    Protean Plus DodecaCell (Bio-Rad)
    Ettan SE600 Ruby gel electrophoresis system with auto gel stainer
    Ettan DALTtwelve, running 12 large format gels in parallel

    Imaging
    PharosFX (Bio-Rad)
    EXQuest automated spotcutter (Bio-Rad)
    PDQuest (Bio-Rad)
    Image Master 2D scanner and software

    HPLC
    1100 nanoHPLC (Agilent Technologies)

    Mass spectrometry
    MAPII AutoPrep MALDI Robot system (Bruker Daltonics)
    Bruker autoflex® Daltonics MALDI-TOF mass spectrometer with post-source decay
    QStar Elite (ESI/MALDI) (Applied Biosystems)
    Qtrap (ESI) (Applied Biosystems)

    Protein-interaction
    Biocore 3000 (GE Amersham)

    Services:

    Proteomics services are available to internal and external customers. The following services are available:

    Biomarker & Drug-target discovery and validation

    Gel-based proteomics

    Solution-based (gel-free) proteomics

    • Off-line and on-line fractionation:
    • MRM-based During ‘validation’, putative targets are confirmed by a targeted approach where fewer analytes are monitored in a larger sample size. The main aim of this stage is to assess marker sensitivity (likelihood that an affected/diseased sample will test positive). In ‘verification’ the analysis is further extended with 100s of samples analysed in order to examine variations due to environmental, genetic and/or biological factors. Thus, candidate biomarker sensitivity is affirmed while specificity (likelihood that an unaffected/healthy sample will test negative) is gauged.”
    • Mass spectrometry (iTRAQTM, peptide identification, peptide sequence identification, glycan analysis, posttranslational modification analysis)
    • Protein characterisation (protein-protein / ligand interaction)

    Microarrays (cDNA oligo)

    •     Sample preparation and hybridisation
    •     Conventional and high-resolution scanning*
    •     Expression analysis
    •     Quantitative Real-Time PCR

    Contact information
    Dr Stoyan Stoychev

    Molecular Technologies

    Tel: +27 12 841 2270/3001

    Fax: +27 12 841 2388

    Email: az.oc1569109295.risc1569109295@vehc1569109295yotss1569109295