THE ACGT DATABASE

The ACGT database is a compilation of biosciences research skills, techniques and specialised equipment of scientists at the University of Witwatersrand, University of Pretoria, CSIR, University of Johannesburg and the Agricultural Research Council. ACGT is instrumental in creating an awareness of the regional research activities and programmes. It was created to stimulate coordination of research efforts between the partner institutions and provide a platform for future collaborations.

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Personal Information

First NameThomas
Last NameAtkinson
TitleMr
E-Mail Addresstatkinso@csir.co.za
Department / InstitutionBiochemistry, University of Pretoria
Faculty
Address 1
Address 2
Address 3
Address 4
Telephone Number
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Web Site
Web link to CV

Associated Equipment

Equipment Name Equipment Location Equipment Facility Department Responsible Person

Associated Research Programmes

Research Programme Name Techniques Keywords Research Field Responsible Person
HIV Pathogenesis Research Lab RT-PCR, Cloning into bacterial, baculovirus and mammalian expression vectors, Recombinant protein expression in bacterial, insect, mammalian cell lines, and purification, SPR, Chromatography, HPLC, PBMC isolations and co-cultures, HIV culture, Establishing TCID50 Determining drug IC50 against HIV isolates, Circular diachroism HIV-1 Subtype C, subtyping, envelope glycoprotein, CCR5-utilizing virus, CXCR4-utilizing virus, vif, vpr, vpu, envelope-mediated apoptosis, inhibition assays, neutralizing antibody assays, PBMC isolation and co-cultures, pseudovirions, protein binding kinetics, structure and conformation analysis, Biochemistry, molecular biology, protein biochemistry, molecular virology HIV Atkinson, Thomas